Production and characterization of a bioflocculant produced by Aspergillus flavus

► Cation-independent bioflocculant produced by Aspergillus flavus. ► Sucrose and peptone are the most favorable sources for bioflocculant production. ► The bioflocculant mainly consists of polysaccharide and protein with a molecular weight of 2.57 × 104 Da. ► The bioflocculant is stable at wide ranges of pH and temperature.