Biological pretreatment for production of lignocellulosic biofuel

► Lignocellulosic biomass (LCB) was submitted to a biological pretreatment under controlled conditions. ► Organic matter was characterised during the biodegradation process. ► Analysis of lipids and thermochemolysis were used to monitor bacterial activity. ► Humic/fulvic acids ratio, IR and TG analysis traduced OM complexification. ► Biodegradation improved the quality and the quantity of bio-oil.