کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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682116 | 888975 | 2010 | 7 صفحه PDF | دانلود رایگان |

Transgenic tobacco plants expressing the hyperthermostable β-glucosidase BglB of Thermotoga maritima were generated with the goal of cost-effective production of the enzyme for the application in bioconversion of lignocellulosic biomass. The enzyme was targeted to the cytosol and chloroplasts, where it accumulated to level of 4.5% and 5.8% of total soluble protein, respectively. The optimal temperature and pH of the plant-expressed BglB was 80 °C and 4.5, respectively. BglB activity was preserved in leaves after lyophilization, but decreased by over 70% with drying at room temperature. When BglB was synergistically supplied in a 1% (w/v) rice straw with Cel5A for efficient cellulase conversion, a 37% increase in glucose was observed. This report demonstrates the potential of utilizing transgenic tobacco for mass production of BglB.
Journal: Bioresource Technology - Volume 101, Issue 18, September 2010, Pages 7144–7150