کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
682116 888975 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression of thermostable bacterial β-glucosidase (BglB) in transgenic tobacco plants
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Expression of thermostable bacterial β-glucosidase (BglB) in transgenic tobacco plants
چکیده انگلیسی

Transgenic tobacco plants expressing the hyperthermostable β-glucosidase BglB of Thermotoga maritima were generated with the goal of cost-effective production of the enzyme for the application in bioconversion of lignocellulosic biomass. The enzyme was targeted to the cytosol and chloroplasts, where it accumulated to level of 4.5% and 5.8% of total soluble protein, respectively. The optimal temperature and pH of the plant-expressed BglB was 80 °C and 4.5, respectively. BglB activity was preserved in leaves after lyophilization, but decreased by over 70% with drying at room temperature. When BglB was synergistically supplied in a 1% (w/v) rice straw with Cel5A for efficient cellulase conversion, a 37% increase in glucose was observed. This report demonstrates the potential of utilizing transgenic tobacco for mass production of BglB.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 101, Issue 18, September 2010, Pages 7144–7150
نویسندگان
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