کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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682660 | 888989 | 2010 | 7 صفحه PDF | دانلود رایگان |
Saccharomyces cerevisiae JUC15 was successfully obtained by target reaction-oriented screening, which reduced 2-hydroxy-1-phenylethanone (HPE) to (R)-phenyl-1,2-ethanediol ((R)-PED) of excellent enantiomeric excess (e.e. >99.9%). There was no significant decrease in the yield and optical purity of (R)-PED when the free cells were reused for 40 repeated cycles at 2 g L−1 substrate concentration. The strain used cheap sucrose for cofactor regeneration and worked over a considerably wider range of pH (4–9). The optimum substrate concentration was 8 g L−1, which was higher than any other biocatalysts reported so far. Interesting, when HPE concentration reached 20 g L−1 in reaction system, where 43.2% of the substrate was present in suspended solid form, the reaction still gave enantiopure (R)-PED in 71% yield. Last but not least, the product e.e. kept above 99.9% in all examined conditions. These results suggest the potential of this strain for the industrial production of optically active (R)-PED.
Journal: Bioresource Technology - Volume 101, Issue 22, November 2010, Pages 8502–8508