کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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683108 | 888995 | 2010 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Identification of nitrile hydratase-producing Rhodococcus ruber TH and characterization of an amiE-negative mutant Identification of nitrile hydratase-producing Rhodococcus ruber TH and characterization of an amiE-negative mutant](/preview/png/683108.png)
Microbial transformation of acrylonitrile to acrylamide by nitrile hydratase is of great interest to green chemistry. During the transformation, acrylic acid is generally accumulated as a by-product through biocatalysis of amidase. A novel strain with high nitrile hydratase activity was isolated from soil and identified as Rhodococcus ruber TH by morphology and 16S rRNA gene analysis. An amidase-negative recombinant, R. ruber TH3 was constructed. Its nitrile hydratase activity was 25% higher than that of the wild type, reaching 490 ± 29.2 U/mg dry cell weight, while the amidase activity was 60% lower. After 6 h hydration of acrylonitrile using free cells as biocatalysts at 18 °C, acrylamide production by TH3 was 23% higher and the production of the by-product, acrylic acid, was 87% lower than that of the wild type. This result demonstrates that the strain TH3 could be valuable for industrial applications.
Journal: Bioresource Technology - Volume 101, Issue 1, January 2010, Pages 285–291