کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
683175 888996 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of NH4+ and glycerol on cytidine 5′-diphosphocholine synthesis in Saccharomyces cerevisiae
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Effect of NH4+ and glycerol on cytidine 5′-diphosphocholine synthesis in Saccharomyces cerevisiae
چکیده انگلیسی

Both stimulation of ammonium ion on the glycolytic flux and regulation by glycerol of enzymes in Kennedy pathway for cytidine diphosphate choline production in S. cerevisiae were studied. The conventional transformation course featured four stages. Firstly, CMP and choline chloride were phosphorylated and CDP-choline was formed rapidly; secondly, the rate of CDP-choline formation declined and CMP was not detected in the mixture; thirdly, CMP was released and the CDP-choline concentration reached a peak; Fourthly, the compound concentrations did not practically changes eventually. Using the central composite design, the concentration, yield, and utilization efficiency of energy reached 24.7 mmol/L, 82.3% and 10.6%, with 30 mmol/L of ammonium ion and 1% (V/V) of glycerol, respectively. Ammonium ion not only strengthened the glycolytic pathway, but also coordinated the reaction rate between the glycolytic pathway and the Kennedy pathway. Glycerol alleviated the activity decrease of the key enzymes in the mixture.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 100, Issue 20, October 2009, Pages 4848–4853
نویسندگان
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