کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
683221 | 888997 | 2010 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Improvement in fruiting body yield by introduction of the Ampullaria crossean multi-functional cellulase gene into Volvariella volvacea
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Improvement in fruiting body yield by introduction of the Ampullaria crossean multi-functional cellulase gene into Volvariella volvacea Improvement in fruiting body yield by introduction of the Ampullaria crossean multi-functional cellulase gene into Volvariella volvacea](/preview/png/683221.png)
چکیده انگلیسی
The multi-functional cellulase gene, mfc, from Ampullaria crossean was transformed into Volvariella volvacea by PEG-mediated protoplast transformation to improve the biological efficiency and fruiting body yield. PCR and Southern blotting indicated that mfc was integrated into the genomes of four transformants. In laboratory and large scale cultivation experiments, the average biological efficiency of the transformants was 18.39 ± 1.27% and 27.84 ± 3.21%, respectively, considerably higher than the corresponding values for untransformed controls of 12.69 ± 1.31% and 20.63 ± 2.59%. This is the first report of an improvement in biological efficiency and fruiting body yield of V. volvacea through transgenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 101, Issue 16, August 2010, Pages 6482–6486
Journal: Bioresource Technology - Volume 101, Issue 16, August 2010, Pages 6482–6486
نویسندگان
Feng-Yun Zhao, Jun-Fang Lin, Xian-Lu Zeng, Li-Qiong Guo, Yi-Hong Wang, Li-Rong You,