کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
683286 888998 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An alkaline lipase from organic solvent tolerant Acinetobacter sp. EH28: Application for ethyl caprylate synthesis
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
An alkaline lipase from organic solvent tolerant Acinetobacter sp. EH28: Application for ethyl caprylate synthesis
چکیده انگلیسی
A mesophilic bacterium producing a thermostable alkaline lipase was isolated from oil rich soil sample and identified as Acinetobacter sp. EH28. The lipase was partially purified by ammonium sulphate precipitation followed by hydrophobic interaction chromatography with 24.2-fold purification and 57.1 U/ml specific activity. The partially purified enzyme exhibited maximum activity at pH 10.0 and at 50 °C and was highly stable at 50 °C retaining 100% of its activity up to 90 min. It was highly stable and retained more than 80% of its initial activity upon exposure to various organic solvents. The EH28 lipase was used for synthesis of the flavor ester ethyl caprylate in organic solvents, thus providing a concept of application of Acinetobacter sp. lipase in non-aqueous catalysis. Reaction parameters best suited for this esterification reaction were 40 °C reaction temperature, 1.3:1 ratio of caprylic acid to ethanol and cyclohexane as the medium.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 101, Issue 10, May 2010, Pages 3628-3634
نویسندگان
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