کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
683321 | 888999 | 2009 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Biocatalytic cascade oxidation using laccase for pyranose 2-oxidase regeneration Biocatalytic cascade oxidation using laccase for pyranose 2-oxidase regeneration](/preview/png/683321.png)
The interactions between two oxidoreductases coupled by an artificial redox mediator have been described quantitatively to increase both stability and productivity. In this cascade oxidation, pyranose 2-oxidase oxidizes several aldoses at the C-2 position to 2-ketoaldoses. A redox mediator is used as electron acceptor for pyranose 2-oxidase because it shows more favourable kinetics in comparison to oxygen. The reduced redox mediator is in turn re-oxidized by laccase, which uses oxygen as the terminal electron acceptor, reducing it fully to water. However, pyranose 2-oxidase is capable of using oxygen as an electron acceptor in a competing side reaction, leading to the formation of hydrogen peroxide, which is detrimental for both enzymes and seriously limits the operational stability of both enzymes.The experimental results showed full conversion of the aldose to the 2-ketoaldose and a good agreement with the simulations of the process.
Journal: Bioresource Technology - Volume 100, Issue 23, December 2009, Pages 5566–5573