کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
683510 889001 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Optimization of process parameters for the production of tyrosine phenol lyase by Citrobacterfreundii MTCC 2424
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Optimization of process parameters for the production of tyrosine phenol lyase by Citrobacterfreundii MTCC 2424
چکیده انگلیسی

The process optimization using technological combinations for the production of tyrosine phenol lyase by Citrobacter freundii MTCC 2424 has been carried out in this study. The maximum production of tyrosine phenol lyase (0.15 U) was obtained by culturing C. freundii MTCC 2424 in a medium containing (g/l) meat extract 5.0, yeast extract 5.0, peptone 2.5, and l-tyrosine 1.0 at 25 °C for 16 h in a temperature controlled orbital shaker. A 2.5-fold increase in enzyme activity with 1.3-fold decrease in the cost of enzyme production (in terms of media components) was achieved by using different technological combinations. The process optimization using technological combinations allowed quick optimization of large number of variables, which helps in designing of suitable fermentation conditions for the cost-effective production of tyrosine phenol lyase. Moreover, this also provides information for balancing the nutrient concentration with minimum experimentation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 100, Issue 5, March 2009, Pages 1840–1846
نویسندگان
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