کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
684547 889023 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Optimization of media composition for Nattokinase production by Bacillus subtilis using response surface methodology
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Optimization of media composition for Nattokinase production by Bacillus subtilis using response surface methodology
چکیده انگلیسی

Response surface methodology and central composite rotary design (CCRD) was employed to optimize a fermentation medium for the production of Nattokinase by Bacillus subtilis at pH 7.5. The four variables involved in this study were Glucose, Peptone, CaCl2, and MgSO4. The statistical analysis of the results showed that, in the range studied; only peptone had a significant effect on Nattokinase production. The optimized medium containing (%) Glucose: 1, Peptone: 5.5, MgSO4: 0.2 and CaCl2: 0.5 resulted in 2-fold increased level of Nattokinase (3194.25 U/ml) production compared to initial level (1599.09 U/ml) after 10 h of fermentation. Nattokinase production was checked with fibrinolytic activity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 99, Issue 17, November 2008, Pages 8170–8174
نویسندگان
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