کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
684547 | 889023 | 2008 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Optimization of media composition for Nattokinase production by Bacillus subtilis using response surface methodology
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Response surface methodology and central composite rotary design (CCRD) was employed to optimize a fermentation medium for the production of Nattokinase by Bacillus subtilis at pH 7.5. The four variables involved in this study were Glucose, Peptone, CaCl2, and MgSO4. The statistical analysis of the results showed that, in the range studied; only peptone had a significant effect on Nattokinase production. The optimized medium containing (%) Glucose: 1, Peptone: 5.5, MgSO4: 0.2 and CaCl2: 0.5 resulted in 2-fold increased level of Nattokinase (3194.25 U/ml) production compared to initial level (1599.09 U/ml) after 10 h of fermentation. Nattokinase production was checked with fibrinolytic activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 99, Issue 17, November 2008, Pages 8170–8174
Journal: Bioresource Technology - Volume 99, Issue 17, November 2008, Pages 8170–8174
نویسندگان
V. Deepak, K. Kalishwaralal, S. Ramkumarpandian, S. Venkatesh Babu, S.R. Senthilkumar, G. Sangiliyandi,