کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
684760 | 889027 | 2008 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Effects of select nitrocompounds on in vitro ruminal fermentation during conditions of limiting or excess added reductant Effects of select nitrocompounds on in vitro ruminal fermentation during conditions of limiting or excess added reductant](/preview/png/684760.png)
Ruminal methane (CH4) production results in the loss of up to 12% of gross energy intake and contributes nearly 20% of the United States’ annual emission of this greenhouse gas. We report the effects of select nitrocompounds on ruminal fermentation after 22 h in vitro incubation (39 °C) with or without additions of hydrogen (H2), formate or both. In incubations containing no added reductant, CH4 production was inhibited 41% by 2-nitro-1-propanol (2NPOH) and >97% by 3-nitro-1-propionic acid (3NPA), nitroethane (NE) and 2-nitroethanol (2NEOH) compared to non-treated controls and H2 did not accumulate. With formate as the sole added reductant, nitro-treatment reduced CH4 production by >99% and caused 42% to complete inhibition of formate catabolism compared to controls, and the accumulation of H2 increased slightly. Nitro-treatment decreased CH4 production 57–98% from that of controls when supplied H2 or formate plus H2. Formate catabolism was decreased 42–84% from that in controls by all nitro-treatments except 3NPA with both formate and H2. Greater than 97% of the added H2 was catabolized within controls; >84% was catabolized in nitro-treated incubations. Acetate, propionate and butyrate accumulations were unaffected by nitro-treatment irregardless of reductant; however, effects on ammonia and branched chain fatty acid accumulations varied. These results suggest that nitro-treatment inhibited formate dehydrogenase/formate hydrogen lyase and hydrogenase activity.
Journal: Bioresource Technology - Volume 99, Issue 18, December 2008, Pages 8655–8661