کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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684999 | 889031 | 2009 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Optimization of growth media for obtaining high-cell density cultures of halophilic archaea (family Halobacteriaceae) by response surface methodology Optimization of growth media for obtaining high-cell density cultures of halophilic archaea (family Halobacteriaceae) by response surface methodology](/preview/png/684999.png)
Optimization of media components for the growth and biomass production of Halobacterium salinarum VKMM 013 was carried out using response surface methodology. A second order quadratic model was estimated and media components were determined based on quadratic regression equation generated by model. These were 6.35 g L−1 of KCl, 9.70 g L−1 of MgSO4, 13.38 g L−1 of gelatin and 12.00 g L−1 of soluble starch in nutrient broth supplemented with artificial seawater with 20% (w/v) of NaCl. In these optimal conditions, the obtained cell concentration of 0.746 g L−1 dry weight was in agreement with the predicted cell concentration. The optimized media significantly shortened the time required for cell culture to reach the stationary phase while providing a nearly 2.4-fold increase in biomass production. Furthermore, in cell cultures of three other halophilic archaea the use of optimized media enhanced growth rate and provided high-cell density.
Journal: Bioresource Technology - Volume 100, Issue 12, June 2009, Pages 3107–3112