کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
685007 889031 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Regulation of environmental factors on the expression of a solid-state specific lipase (Lip1) with Rhizopus chinensis by western blot and indirect Elisa
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Regulation of environmental factors on the expression of a solid-state specific lipase (Lip1) with Rhizopus chinensis by western blot and indirect Elisa
چکیده انگلیسی

To identify which solid-state typical environmental factors are involved in the induction of a solid-state special lipase (Lip1), western blot and Elisa based on Lip1 antibody were used. A low water activity played a significant role in the induction of Lip1, as evidenced by the increased expression level (20–46 μg/g dry cell) along with the decrease of water activity (0.927–0.969). Physical barrier against hyphal extension was found to be another required factor, since the expression of Lip1 was significantly enhanced by 3-fold using a membrane with smaller pore size (0.45 and 0.22 μm) covered on top of surface culture.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 100, Issue 12, June 2009, Pages 3152–3156
نویسندگان
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