کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
685332 | 889037 | 2008 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Fermentation characteristics of Fusariumoxysporum grown on acetate Fermentation characteristics of Fusariumoxysporum grown on acetate](/preview/png/685332.png)
In this study, the growth characteristics of Fusariumoxysporum were evaluated in minimal medium using acetate or different mixtures of acetate and glucose as carbon source. The minimum inhibitory concentration (MIC) of acetic acid that F.oxysporum cells could tolerate was 0.8% w/v while glucose was consumed preferentially to acetate. The activity of isocitrate lyase was high when cells were grown on acetate and acetate plus glucose indicating an activation of the glyoxylate cycle. Investigation of the metabolic fingerprinting and footprinting revealed higher levels of intracellular and extracellular TCA cycle intermediates when F.oxysporum cells were grown on mixtures of acetate and glucose compared to growth on only glucose. Our data support the hypothesis that a higher flux through TCA cycle during acetate consumption could significantly increase the pool of NADH, resulting in the activation of succinate–propionate pathway which consumes reducing power (NADH) via conversion of succinate to propionyl-CoA and produce propionate.
Journal: Bioresource Technology - Volume 99, Issue 15, October 2008, Pages 7397–7401