کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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685783 | 889049 | 2008 | 8 صفحه PDF | دانلود رایگان |
This work investigates the potential of metabolic stimulators, firstly to enhance the production of β-carotene, and later use of inhibitors of lycopene cyclase so as to accumulate lycopene in the fermentation medium. Various non-ionic surfactants, natural oils, stimulators such as amino-acids, tricarboxylic acid cycle (TCA) intermediates, vitamin A and antibiotics were investigated for improved production of β-carotene using the zygomycete fungus Blakeslea trispora. Span 20 at 0.2% increased the β-carotene production from 139 mg/l to 318 mg/l. Examination of the mycelial morphology of the B. trispora with span 20 showed a shorter mycelial length, which allowed a well-dispersed growth of B. trispora. Supplementation of the medium with 1000 ppm vitamin A acetate gave highest concentration of β-carotene (830 ± 6 mg/l). Several chemical inhibitors such as imidazole, pyridine, triethylamine, piperidine, and nicotinic acid were then evaluated to block the biosynthesis at lycopene. Piperidine at 500 ppm gave a 7.76-fold improvement, and produced high titers of lycopene (775 ± 5 mg/l) in a medium supplemented with vitamin A acetate.
Journal: Bioresource Technology - Volume 99, Issue 8, May 2008, Pages 3166–3173