کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
686250 889066 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Manganese peroxidase production in submerged cultures by free and immobilized mycelia of Nematoloma frowardii
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Manganese peroxidase production in submerged cultures by free and immobilized mycelia of Nematoloma frowardii
چکیده انگلیسی

The agaric basidiomycete Nematoloma frowardii has been suggested as a good alternative for production of the extracellular ligninolytic enzyme, manganese-dependent peroxidase (MnP). Some cultural and environmental factors influencing the enzymatic activity in shaken flasks and aerated fermenter cultures were evaluated to improve the yields of the process. A low nitrogen medium (1.36 mM N added as ammonium tartrate), containing 16 g/l glucose (C/N ratio = 65.3), 2 mM Mn2+ and inoculated with immobilized polyurethane foam mycelium, made it possible to obtain a MnP yield of 2304 nkat/l in 8 days. Under these operational conditions, the enzyme productivity in the immobilized cells of N. frowardii was 1.4 times higher than that obtained with the free fungus. In the procedure with the reusable immobilized mycelium (semi-continuous culture) as many as three subsequent 10 day batches could be fermented by using the same carrier with no loss of MnP activity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 97, Issue 3, February 2006, Pages 469–476
نویسندگان
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