کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
686659 889100 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An extracellular—Pepstatin insensitive acid protease produced by Thermoplasma volcanium
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
An extracellular—Pepstatin insensitive acid protease produced by Thermoplasma volcanium
چکیده انگلیسی

In this study, some parameters for the production and caseinolytic activity of an extracellular thermostable acid protease from a thermoacidophilic archaeon Thermoplasma volcanium were determined. The highest level of growth and enzyme production were detected at pH 3.0 over an incubation period of 192 h at 60 °C. The pH optimum for the acid protease activity was 3.0 and the enzyme was fairly stable over a broad pH range (pH 3.0–8.0). The temperature for maximum activity of the enzyme was 55 °C and activity remained stable between 50 °C and 70 °C. These features could be of relevance for various biotechnological applications of this enzyme. Serine-(PMSF), cysteine-(DTT), metallo-(EDTA) and aspartate-(pepstatin) protease inhibitors did not inhibit the caseinolytic activity of the enzyme. Therefore, Tp. volcanium acid protease could be a member of the pepstatin-insensitive carboxyl proteinases.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 98, Issue 1, January 2007, Pages 112–117
نویسندگان
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