Optimization of operating parameters for anti-corrosive biopolymer production by Chryseobacterium Indologenes MUT.2 using central composite design methodology

• Application of central composite design for optimization of operating conditions.
• A novel bacterium, C. Indologenes MUT.2, is used to synthesize the biopolymer.
• Study of the correlation between concentrations of biomass and produced biopolymer.
• The importance of an optimization process for biopolymer production is shown.

This study describes the optimized conditions for using the mesophile bacterial strain Chryseobacterium Indologenes MUT.2 to obtain the maximum amount of biomass and biopolymer. The operating conditions in this study were pH = 5–9, temperature = 26–45 °C, and agitation = 70–210 rpm in a shaker flask. Furthermore, response surface methodology was applied to navigate the experimental data in accordance with central composite design. The optimal operating conditions were found to be pH = 7.7, temperature = 33 °C and agitation = 170 rpm. Under these conditions, the maximum biopolymer and biomass concentrations were obtained, i.e. 14.50 g/L and 2.00 g/L, respectively. Statistical results showed an adjusted-R2 of 0.9839 and 0.9313 for biopolymer and biomass concentrations, respectively. Moreover, analysis of variance demonstrated that pH–pH and temperature–temperature effects were found to be the most influential factors for biomass and biopolymer concentrations.

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