Flow cytometry analysis using at the poly(3-hydroxybutyrate-co-3-hydrovalerate) microspheres for drug delivery system

Microspheres can be prepared simply by the W1/O/W2 double emulsion-solvent evaporation method. The glycerol density gradient method can be utilized with a homogenizer to produce and separate out homogenized microspheres, with the desired size. Flow cytometer is utilized to measure not only the uniformity of the size of microspheres but also analytical instruments are used to measure their release of drugs. The diameters of microsphere reached the 5 μm–10 μm range when they were prepared with 1% (w/v) P(3HB-co-5%3HV) at a homogenizer speed of 3000 rpm. The microspheres that were separated using the glycerin gradient method and extracted in the 25% concentration zone reveal the size homogeneity determined by flow cytometry analysis. The best microsphere degradation rates were approximately 80% after 72 h of enzyme degradation, as measured using a spectrophotometer, to elucidate the drug-releasing effect of the microspheres. The concentration of trypan blue in the supernatant fluid increased with the duration of the experiment. P(3HB-co-5%3HV)-prepared microspheres with diameters of around 10 μm were engulfed by cells in the cell culture experiments.

► Poly(3-hydroxybutyrate-co-5%3-hydroxyvalerate) (P3HB-co-5%3HV) micro-spheres can be prepared by W1/O/W2 emulsion solvent evaporation method.
► The glycerol density gradient method can be utilized with a homogenizer to produce and separate out homogenized microspheres, with the desired size.
► Flow cytometer is utilized to measure not only the uniformity of the size of microspheres but also analytical instruments are used to measure their release of drugs.
► The best microsphere degradation rates were approximately 80% after 72 h of enzyme degradation.