The effect of culture conditions on the growth of T. lactinea and anti-inflammatory activities via in vitro inhibition of hyaluronidase and lipoxygenase enzyme activities

• Culture conditions for T. lactinea growth and its biological activities were investigated.
• T. lactinea extract grown in Medium 2 inhibited highest anti-inflammatory enzymes.
• Metabolite profile in the T. lactinea extract was analyzed using TLC and HPLC.
• Rutin in the fungus extract affected inhibition of anti-inflammatory enzyme activities.

Trametes lactinea (T. lactinea) is another macrofungus that exists in the Malaysian tropical forest, but information about the fungus is very scarce in the literature. Effect of culture conditions on the growth of T. lactinea and anti-inflammatory activities via in vitro inhibition of hyaluronidase and lipoxygenase enzyme by its culture broth extract was investigated. Our findings showed that culture conditions such as media composition, pH of the culture medium, inoculum volume, incubation temperature and incubation time affected the growth and anti-inflammatory activities. The highest hyaluronidase (92.5 ± 0.86%) and lipoxygenase (93.5 ± 0.49%) enzyme inhibitions were obtained from the extract incubated in Medium 2, pH 6, 5 mL of inoculum volume at 35 °C for 8 days. Medium 2 was found as unfavorable for the growth of T. lactinea. TLC, UV–vis spectrophotometer and HPLC analysis found that rutin was presence in the fungus extract.