کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
691996 1460463 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Supercritical fluids extraction of cinnamic acid derivatives from Brazilian propolis and the effect on growth inhibition of colon cancer cells
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Supercritical fluids extraction of cinnamic acid derivatives from Brazilian propolis and the effect on growth inhibition of colon cancer cells
چکیده انگلیسی
This work examined supercritical carbon dioxide (SC-CO2) extractions of an anti-cancer compound, 3,5-diprenyl-4-hydroxycinnamic acid (DHCA) from propolis lumps and studied the inhibitive effect of the SC-CO2 extracts on the growth of two cancer cells. The maximum amount of DHCA, 91.9 mg/g, was obtained by Soxhlet ethyl acetate extraction and the 41.2 wt.% pure DHCA was recovered using SC-CO2 at 20.7 MPa and 323 K with 6 wt.% ethyl acetate addition. The effects of temperature and the addition of ethyl acetate on the DHCA purity, examined using a two factorial central composite response surface methodology, indicated that both factors were significant. A normal phase column adsorption approach was directly employed to purify the DHCA, and the purity was increased to 95 wt.%. The cell concentrations following growth inhibition ranged from 10 μg/mL to 500 μg/mL, indicating that the SC-CO2 extracts and 95 wt.% DHCA effectively inhibited the growth of human leukemia (HL-60) and colon (colo 205) cancer cells, but did not suppress the growth of two human normal cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the Taiwan Institute of Chemical Engineers - Volume 40, Issue 2, March 2009, Pages 130-135
نویسندگان
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