کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
691996 | 1460463 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Supercritical fluids extraction of cinnamic acid derivatives from Brazilian propolis and the effect on growth inhibition of colon cancer cells
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
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چکیده انگلیسی
This work examined supercritical carbon dioxide (SC-CO2) extractions of an anti-cancer compound, 3,5-diprenyl-4-hydroxycinnamic acid (DHCA) from propolis lumps and studied the inhibitive effect of the SC-CO2 extracts on the growth of two cancer cells. The maximum amount of DHCA, 91.9 mg/g, was obtained by Soxhlet ethyl acetate extraction and the 41.2 wt.% pure DHCA was recovered using SC-CO2 at 20.7 MPa and 323 K with 6 wt.% ethyl acetate addition. The effects of temperature and the addition of ethyl acetate on the DHCA purity, examined using a two factorial central composite response surface methodology, indicated that both factors were significant. A normal phase column adsorption approach was directly employed to purify the DHCA, and the purity was increased to 95 wt.%. The cell concentrations following growth inhibition ranged from 10 μg/mL to 500 μg/mL, indicating that the SC-CO2 extracts and 95 wt.% DHCA effectively inhibited the growth of human leukemia (HL-60) and colon (colo 205) cancer cells, but did not suppress the growth of two human normal cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the Taiwan Institute of Chemical Engineers - Volume 40, Issue 2, March 2009, Pages 130-135
Journal: Journal of the Taiwan Institute of Chemical Engineers - Volume 40, Issue 2, March 2009, Pages 130-135
نویسندگان
Chao-Rui Chen, Ying-Nong Lee, Miau-Rong Lee, Chieh-Ming J. Chang,