Driving forces for the adsorption of a His-tag Chagas antigen. A rational approach to design bio-functional surfaces

- Electrostatic and hydrophobic interactions drive physical adsorption.
- Bio-affinity interactions control the His6-H49-Ni(II) behavior.
- Physical adsorption is partially removed with NaCl.
- Surface bioactivity studied by a direct, non-labeled electrochemical detection method.
- Surface bioactivity caused by site-oriented antigen due to bio-affinity interaction.