Atomic force microscopy studies of the influence of convex and concave nanostructures on the adsorption of fibronectin

► The adsorption of fibronectin on a grooved nanostructure was investigated. ► At low concentrations fibronectin preferentially adsorbed in the grooves. ► The amount of adsorbed protein per surface area was increased on the nanostructure. ► The increased protein density led to a higher degree of native fibronectin. ► At high concentrations fibronectin was uniformly distributed across the topography.