کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7066053 | 1459877 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Increased poly-β-hydroxybutyrate production from carbon dioxide in randomly mutated cells of cyanobacterial strain Synechocystis sp. PCC 6714: Mutant generation and characterization
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
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چکیده انگلیسی
Photosynthetic Poly-β-hydroxybutyrate (PHB) productivity in cyanobacteria needs to be increased to make cyanobacterial derived bioplastics economically feasible and competitive with petroleum-based plastics. In this study, high PHB yielding mutants of Synechocystis sp. PCC 6714 have been generated by random mutagenesis, using UV light as a mutagen. The selection of strains was based on PHB content induced by nitrogen and phosphorus starvation. The fast growing mutant MT_a24 exhibited more than 2.5-fold higher PHB productivity than that of the wild-type, attaining values of 37â¯Â±â¯4% dry cell weight PHB. The MT_a24 was characterized for phenotypes, CO2 uptake rate and gene expression levels using quantitative PCR. Genome sequencing showed that UV mutagenesis treatment resulted in a point mutation in the ABC-transport complex, phosphate-specific transport system integral membrane protein A (PstA). The MT_a24 shows potential for industrial production of PHB and also for carbon capture from the atmosphere or point sources.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 266, October 2018, Pages 34-44
Journal: Bioresource Technology - Volume 266, October 2018, Pages 34-44
نویسندگان
Donya Kamravamanesh, Tamas Kovacs, Stefan Pflügl, Irina Druzhinina, Paul Kroll, Maximilian Lackner, Christoph Herwig,