کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7066089 | 1459877 | 2018 | 34 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biosynthesis of pyruvic acid from glycerol-containing substrates and its regulation in the yeast Yarrowia lipolytica
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
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چکیده انگلیسی
The ability of different yeasts to synthesize pyruvic acid (PA) from glycerol-containing substrates has been studied. The selected strain Yarrowia lipolytica VKM Y-2378 synthesized PA with α-ketoglutaric acid (KGA) as a byproduct. The content of KGA greatly depended on cultivation conditions. The minimal formation of the byproduct was provided by the limitation of yeast growth by thiamine (0.6â¯Âµg/g biomass); the use of ammonium sulfate (0.6%) as a nitrogen source; addition of glycerol to cultivation medium in 20â¯g/L portions; maintaining the cultivation temperature at 28â¯Â°C, pH of the cultivation medium at 4.5, and medium aeration between 55 and 60% of saturation; the optimal cultivation time was 48â¯h. The selected strain cultivated under such conditions in a fermenter with a waste glycerol from biodiesel production process synthesized 41â¯g/L PA with a yield of 0.82â¯g/g. The mechanism of PA production from glycerol-containing substrates in Y. lipolytica is discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 266, October 2018, Pages 125-133
Journal: Bioresource Technology - Volume 266, October 2018, Pages 125-133
نویسندگان
Svetlana V. Kamzolova, Igor G. Morgunov,