کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7069203 | 1459896 | 2018 | 35 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Engineering peptidoglycan degradation related genes of Bacillus subtilis for better fermentation processes
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Engineering peptidoglycan degradation related genes of Bacillus subtilis for better fermentation processes Engineering peptidoglycan degradation related genes of Bacillus subtilis for better fermentation processes](/preview/png/7069203.png)
چکیده انگلیسی
In this study, Bacillus subtilis 168 Îupp was engineered to change the bacterial shapes. Namely, some peptidoglycan hydrolase related genes were inactivated individually or in different combinations, including sigD, lytE, lytF, lytC, lytD and lytG. Inactivations of these genes resulted in various intensities of blockages on cell division, leading to elongation of bacterial cells. The resulted fiber phenotypes showed different lengths ranging from tens of microns to several millimeters. Mutants with multiple gene inactivations such as ÎsigDÎlytEÎlytD showed more easily precipitated phenomenon, obviously increased growth rate, more sensitive to antibiotics and improved α-amylase production compared with that of B. subtilis 168 Îupp. Mutants ÎsigDÎlytEÎlytD and ÎsigDÎlytEÎlytCÎlytD also showed an increased tolerance to high osmotic pressure of sodium chloride, allowing unsterile fermentation, all of which contributes to reduced processing cost.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 248, Part A, January 2018, Pages 238-247
Journal: Bioresource Technology - Volume 248, Part A, January 2018, Pages 238-247
نویسندگان
Liang Zhao, Jianwen Ye, Jing Fu, Guo-Qiang Chen,