کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7069246 | 1459893 | 2018 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
d-1,2,4-Butanetriol production from renewable biomass with optimization of synthetic pathway in engineered Escherichia coli
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Bio-based production of d-1,2,4-butanetriol (BT) from renewable substrates is increasingly attracting attention. Here, the BT biosynthetic pathway was constructed and optimized in Escherichia coli to produce BT from pure d-xylose or corncob hydrolysates. First, E. coli BL21(DE3) was identified as a more proper host for BT production through host screening. Then, BT pathway was systematically optimized with gene homolog screening strategy, mainly targeting three key steps from xylonic acid to BT catalyzed by d-xylonate dehydratase (XD), 2-keto acid decarboxylase (KDC) and aldehyde reductase (ALR). After screening six ALRs, four KDCs and four XDs, AdhP from E. coli, KdcA from Lactococcus lactis and XylD from Caulobacter crescentus were identified more efficiently for BT production. The co-expression of these enzymes in recombinant strain BL21-14 led to BT production of 5.1â¯g/L under the optimized cultivation conditions. Finally, BT production from corncob hydrolysates was achieved with a titer of 3.4â¯g/L.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 250, February 2018, Pages 406-412
Journal: Bioresource Technology - Volume 250, February 2018, Pages 406-412
نویسندگان
Xin Wang, Nana Xu, Shewei Hu, Jianming Yang, Qian Gao, Sheng Xu, Kequan Chen, Pingkai Ouyang,