کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7069485 | 1459899 | 2017 | 17 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enhancing linalool production by engineering oleaginous yeast Yarrowia lipolytica
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this study, stepwise increases in linalool production were obtained by combining metabolic engineering and process optimization of an unconventional oleaginous yeast Yarrowia lipolytica. The linalool synthetic pathway was successfully constructed by heterologously expressing a codon-optimized linalool synthase gene from Actinidia arguta in Y. lipolytica. To enhance linalool productivity, key genes involved in the mevalonate pathway were overexpressed in different combinations. Moreover, the overexpression of mutant ERG20F88W-N119W gene resulted in further linalool production. A maximum linalool titre of 6.96 ± 0.29 mg/L was achieved in shake flasks, which was the highest level ever reported in yeasts.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 245, Part B, December 2017, Pages 1641-1644
Journal: Bioresource Technology - Volume 245, Part B, December 2017, Pages 1641-1644
نویسندگان
Xuan Cao, Liu-Jing Wei, Jia-Yu Lin, Qiang Hua,