کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7074656 | 1459951 | 2015 | 35 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enhancement of dalesconols A and B production via upregulation of laccase activity by medium optimization and inducer supplementation in submerged fermentation of Daldinia eschscholzii
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Dalesconols (dalesconols A and B) are novel polyketides with strong immunosuppressive activity produced by Daldinia eschscholzii. In this work, the effects of different media (M1, M2, and M3) on fungus growth and dalesconols biosynthesis were firstly tested and compared. Intermediates and enzyme analysis indicated that laccase had the major contribution to dalesconols biosynthesis. The key role of laccase on dalesconols biosynthesis was further experimentally confirmed, which suggested that the modified M2 was more favored for laccase and dalesconols production. Thereafter, the medium composition was optimized by RSM with a fermentation titer of 36.66Â mg/L obtained. Furthermore, Ca2+ induction was employed to up-regulate of laccase activity and further enhanced dalesconols production (76.90Â mg/L), which was 308% higher than that in M2. In addition, dalesconols production reached 63.42Â mg/L in scale-up experiments. This work indicated great potential of laccase as a key enzyme on regulation of dalesconols production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 192, September 2015, Pages 346-353
Journal: Bioresource Technology - Volume 192, September 2015, Pages 346-353
نویسندگان
Zheng-Hua Pan, Rui-Hua Jiao, Yan-Hua Lu, Ren-Xiang Tan,