Vermicomposting of Tea Factory Coal Ash: Metal accumulation and metallothionein response in Eisenia fetida (Savigny) and Lampito mauritii (Kinberg)

Earthworms can accumulate heavy metals in their intestines to a great extent. Impact of feed materials and duration of metal exposure on natural activity of earthworms are rather unclear; this investigation therefore addresses the impact of metal rich Tea Factory Coal Ash (TFCA) on reproduction, composting and metal accumulation ability of Eisenia fetida and Lampito mauritii. Earthworm count and cocoon production increased significantly during vermicomposting. pH of the vermicomposted mixtures shifted toward neutrality, total organic C decreased substantially and total N enhanced significantly compared to composting. High heavy metal (Mn, Zn, Cu, As) accumulation was recorded in the intestine of both the earthworm species. Moreover, gradual increase in the metal-inducible metallothionein concentration indicated the causal mechanism of metal accumulation in these species. TFCA + cow dung (CD) (1:1) were most favorable feed mixture for E. fetida and TFCA + CD (1:2) were good for L. mauritii in regard to metal accumulation and compost quality.