Boosting accumulation of neutral lipids in Rhodosporidium kratochvilovae HIMPA1 grown on hemp (Cannabis sativa Linn) seed aqueous extract as feedstock for biodiesel production

- Novel oleaginous yeast isolated from Himalayan permafrost soil.
- Hemp seed extract used as cheap triglyceride feedstock for biodiesel production.
- Sonicated HSAE had 10-15% higher lipid yield than GSM and lacks toxic components.
- TAG accumulated supersized lipid droplet of 5.95 ± 1.05 μm visualized by BODIPY stain.
- Unique FAME profile of this yeast showed C20:0, C22:0, C27:0 similar to algal oils.