کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7077700 | 1459978 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Novel thermostable endo-xylanase cloned and expressed from bacterium Geobacillus sp. WSUCF1
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Novel thermostable endo-xylanase cloned and expressed from bacterium Geobacillus sp. WSUCF1 Novel thermostable endo-xylanase cloned and expressed from bacterium Geobacillus sp. WSUCF1](/preview/png/7077700.png)
چکیده انگلیسی
A gene encoding a GH10 endo-xylanase from Geobacillus sp. WSUCF1 was cloned and expressed in Escherichia coli. Recombinant endo-xylanase (37 kDa) exhibited high specific activity of 461.0 U/mg of protein. Endo-xylanase was optimally active on birchwood xylan at 70 °C and pH 6.5. The endo-xylanase was found to be highly thermostable at 50 and 60 °C, retaining 82% and 50% of its original activity, respectively, after 60 h. High xylan conversions (92%) were obtained with oat-spelt xylan hydrolysis. Higher glucan and xylan conversions were obtained on AFEX-treated corn stover with an enzyme cocktail containing WSUCF1 endo-xylanase (71% and 47%) as compared to enzyme cocktail containing commercial fungal endo-xylanase (64% and 41%). High specific activity, active at high pH's, wide substrate specificity, and higher hydrolytic activity on recalcitrant lignocellulose, make this endo-xylanase a suitable candidate for biofuel and bioprocess industries.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 165, August 2014, Pages 314-318
Journal: Bioresource Technology - Volume 165, August 2014, Pages 314-318
نویسندگان
Aditya Bhalla, Kenneth M. Bischoff, Nirmal Uppugundla, Venkatesh Balan, Rajesh K. Sani,