A kinetic study of biohydrogen production from glucose, molasses and cheese whey by suspended and attached cells of Thermotoga neapolitana

- No H2 or O2 inhibition on H2 production by Thermotoga neapolitana.
- Significant substrate (glucose, molasses, cheese whey) inhibition on H2 production.
- With glucose and molasses, biofilms performed better than suspended cells.
- Scale-up of the suspended-cell process to a 19-L fermentor.
- Simulation of the continuous-flow process to evaluate the optimal dilution rate.