کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7081393 1460000 2013 35 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Knockdown of PsbO leads to induction of HydA and production of photobiological H2 in the green alga Chlorella sp. DT
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Knockdown of PsbO leads to induction of HydA and production of photobiological H2 in the green alga Chlorella sp. DT
چکیده انگلیسی
Green algae are able to convert solar energy to H2 via the photosynthetic electron transport pathway under certain conditions. Algal hydrogenase (HydA, encoded by HYDA) is in charge of catalyzing the reaction: 2H+ + 2e− ↔ H2 but usually inhibited by O2, a byproduct of photosynthesis. The aim of this study was to knockdown PsbO (encoded by psbO), a subunit concerned with O2 evolution, so that it would lead to HydA induction. The alga, Chlorella sp. DT, was then transformed with short interference RNA antisense-psbO (siRNA-psbO) fragments. The algal mutants were selected by checking for the existence of siRNA-psbO fragments in their genomes and the low amount of PsbO proteins. The HYDA transcription and the HydA expression were observed in the PsbO-knockdown mutants. Under semi-aerobic condition, PsbO-knockdown mutants could photobiologically produce H2 which increased by as much as 10-fold in comparison to the wild type.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 143, September 2013, Pages 154-162
نویسندگان
, , , , , , ,