Separation and characterization of effective demulsifying substances from surface of Alcaligenes sp. S-XJ-1 and its application in water-in-kerosene emulsion

- Evaluated effects of chemical treatments on demulsifying bacteria.
- Successfully separated effective demulsifying substances from cell surfaces.
- Cell surface substances and structure jointly determined the demulsifying activity.
- Demulsifying substances contained a carbohydrate-protein-lipid complex.
- Demulsifying substances controlled the cell properties related to demulsification.