کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7082639 1460005 2013 31 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of intracellular lipid bodies in Chlamydomonas reinhardtii strains by flow cytometry
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Evaluation of intracellular lipid bodies in Chlamydomonas reinhardtii strains by flow cytometry
چکیده انگلیسی
A comparative study of Chlamydomonas reinhardtii wild type CC124 and a cell wall-less mutant sta6-1 is described using FACS in conjunction with two different lipophilic fluorescent dyes, Nile Red and BODIPY 505/515. The results indicate that BODIPY 505/515 is more effective for the vital staining of intracellular lipid bodies and single cell sorting than Nile Red. While BODIPY 505/515 stained cells continued to grow after single cell sorting using FACS, Nile Red stained cells failed to recover from sorting. In addition, a comprehensive study was performed to establish a quantitative baseline for future studies for either lipid accumulation and/or microalgal growth by measuring various parameters such as cell count, size, fatty acid contents/composition, and optical/confocal images of the wild type and mutant.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 138, June 2013, Pages 30-37
نویسندگان
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