A novel fed-batch process based on the biology of Aurantiochytrium sp. KRS101 for the production of biodiesel and docosahexaenoic acid

► Aurantiochytrium sp. is of considerable interest for docosahexaenoic acid (DHA), and biodiesel. ► Aurantiochytrium biology was investigated, particularly yield coefficients to nutrients. ► Nitrogen is mostly responsible for early stage of cell growth or division. ► Carbon is rather necessary for later stage of cell development. ► With the yield coefficients, modified fed-batch cultivation was successfully performed.