Two-stage cultivation of two Chlorella sp. strains by simultaneous treatment of brewery wastewater and maximizing lipid productivity

► Utilization of wastewater before and after anaerobic digestion as nutrient sources. ► A hybrid system to improve algal biofuel economy with wastewater treatment. ► A new two-stage microalgae cultivation mode to increase lipid productivity. ► Better control on bacterial contamination without any pretreatment.