Highly efficient and regioselective acylation of pharmacologically interesting cordycepin catalyzed by lipase in the eco-friendly solvent 2-methyltetrahydrofuran

► Efficient enzymatic acylation of cordycepin was carried out on 25-g scale. ► Eco-friendly solvent MeTHF was used as a reaction medium for enzymatic acylation. ► Biocatalyst displayed excellent regioselectivity and high operational stability.