Biosynthesis of poly(3-hydroxypropionate) from glycerol by recombinant Escherichia coli

► A P3HP biosynthetic pathway from glycerol was constructed in Escherichia coli. ► Utilization of glucose as cosubstrate improved P3HP production greatly. ► Fermentation process for P3HP production was simplified. ► The highest P3HP production from inexpensive carbon source was achieved.