Continuous butanol fermentation from inexpensive sugar-based feedstocks by Clostridium saccharobutylicum DSM 13864

► Butanol can be produced from cane molasses and corn stover hydrolysate by C. saccharobutylicum. ► A four-stage continuous butanol fermentation using CSH was steadily operated for 220 h. ► A high solvent productivity of 0.429 g/L/h and solvent titer of 11.43 g/L were attained.