Production of succinic acid by engineered E. coli strains using soybean carbohydrates as feedstock under aerobic fermentation conditions

► We engineered E. coli to metabolize soluble sugars derived from soybean to succinate. ► Acid hydrolysate of soy oligosaccharides showed efficient succinate production. ► E. coli strains simultaneously fermented multiple sugars of soybean to succinate. ► 36 g/L succinate produced in aerobic shake flask using soybean feedstock sugars.