Characterization of rhamnolipids produced by non-pathogenic Acinetobacter and Enterobacter bacteria

► Rhamnolipids were produced by non-pathogenic bacteria E. asburiae and A. calcoaceticus. ► These rhamnolipids have mild impact on cell properties of soil bacteria. ► Growth media differing in carbon, nitrogen and phosphorus source were tested. ► Correlation of rhamnolipid fatty acid structure and its properties is indicated.