Statistical optimization of culture media for growth and lipid production of Chlorella protothecoides UTEX 250

- First report of autotrophic medium optimization of Chlorella protothecoides culture.
- This autotrophic cultivation alleviates the contamination in an open pond system.
- A two-stage cultivation strategy was suggested.
- A final biomass concentration of 1.19 g/L was obtained with a 12.9% of lipid content.