Three lignocellulose features that distinctively affect biomass enzymatic digestibility under NaOH and H2SO4 pretreatments in Miscanthus

► Crude- and crystalline cellulose features were distinct on biomass saccharification. ► Crude cellulose CrI/MN and crystalline DP negatively affect biomass digestibility. ► Crude cellulose DP and crystalline MN were positive factors in some cases. ► Cross effects occur among cellulose level and three cellulose features. ► Suggest the potential to genetically engineering of bioenergy crops.