A rapid and consistent near infrared spectroscopic assay for biomass enzymatic digestibility upon various physical and chemical pretreatments in Miscanthus

► Biomass enzymatic digestibility is a crucial parameter for biofuels. ► A rich Miscanthus germplasm allows for a stable and consistent NIRS analysis. ► NIRS assay was performed for biomass digestibility upon various pretreatments. ► Seven optimal equations were finally generated for multiple NIRS predictions. ► NIRS proves as a high-throughput screening tool for biomass enzymatic digestibility.