Deletion of the aroK gene is essential for high shikimic acid accumulation through the shikimate pathway in E. coli

▸ Antisense RNA interference was first employed to inactivate aroK gene. ▸ The aroK gene deletion achieved greater shikimic acid accumulation. ▸ The shikimate kinase activity in the aroK gene deletion strain was lower. ▸ Glycerol is an effective carbon source for shikimic acid accumulation.