Metabolic engineering of Klebsiella oxytoca M5a1 to produce optically pure d-lactate in mineral salts medium

▸ Klebsiella oxytoca was engineered to produce optical pure d-lactate with fewer by-products. ▸ Strains produced d-lactate from sucrose and maltodextrin with impressive yields. ▸ The fermentation was performed in low salt medium under simple anaerobic conditions. ▸ The fermentation conditions are expected to lower cost of d-lactate production. ▸ The costs of downstream processing and waste disposal would be also decreased.