Enhancement of microalgal biomass and lipid productivities by a model of photoautotrophic culture with heterotrophic cells as seed

► The PC-HS model is developed for large-scale biomass and lipid production. ► Rapid cell growth can be achieved in heterotrophic process for seed preparation. ► High biomass and lipid productivity can be achieved in photoautotrophic process. ► The fatty acids compositions of PC-HS are suitable for biofuels production. ► The PC-HS model was also carried out successfully outdoor.